Figure 5: BRAT1 contributes to the expression of RdDM target loci in the RdDM mutants. | Nature Communications

Figure 5: BRAT1 contributes to the expression of RdDM target loci in the RdDM mutants.

From: The Arabidopsis acetylated histone-binding protein BRAT1 forms a complex with BRP1 and prevents transcriptional silencing

Figure 5

(a) The RNA transcripts of AtGP1, AtSN1 and SDC were detected by quantitative RT–PCR in the indicated genotypes. Error bars are s.d. of three technical replicates in a representative experiment. (b) Box plots showing the expression of TEs and protein-coding genes that are downregulated in brat1. FPKM, fragments per kilo base of exons per million fragments mapped. (c) Venn diagrams of overlap between upregulated loci in nrpe1 versus wild type (WT) and downregulated loci in brat1nrpe1 versus nrpe1. (d) Heat maps showing RNA transcripts of upregulated (log2(fold-change of reads)>1, P<0.01,Cufflinks) TEs and genes in nrpe1 compared with the WT. The columns represent the indicated genotypes, and the rows represent the relative RNA transcript levels. Blue indicates low expression, and red indicates high expression.

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