Figure 4: Hyper-long telomeres remain long in stem cell niches and differentiated compartments despite ageing. | Nature Communications

Figure 4: Hyper-long telomeres remain long in stem cell niches and differentiated compartments despite ageing.

From: Generation of mice with longer and better preserved telomeres in the absence of genetic manipulations

Figure 4

(a) Representative image of a 6 months chimaeric intestine after IF with anti-GFP antibody combined with telomere FISH (left). The map shows telomere intensity of each cell, analysed by telomapping. The colour-intensity code is specified over the image (right). (b) Representative image of 6 months chimaeric skin after IF with anti-GFP antibody combined with telomere FISH (left). The map shows telomere intensity for each cell, analysed by telomapping. The colour-intensity code is specified over the image (right). Below, mean telomere-length quantification of the cells contained in the stem cell niches (crypt or hair bulge) and differentiated compartments (villi and basal layer), differentiating the GFP-positive versus GFP-negative patches. Note that longer telomeres coincide with the GFP-positive patches. Representative images of five independent analysis. n=number of chimaeric mice. (c) Magnification of the square area in picture a separating the telomere FISH and the GFP signals. (d) Magnification of the square area in b separating the telomere FISH and the GFP signals. Bar, 20 μm. The s.e.m. was represented in error bars. Student t-test with the Bonferroni correction was used to calculate the P values.

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