Figure 2: Effects of the MDM2 inhibitor nutlin-3a on glucose metabolism and insulin secretion in mice. | Nature Communications

Figure 2: Effects of the MDM2 inhibitor nutlin-3a on glucose metabolism and insulin secretion in mice.

From: The MDM2–p53–pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells

Figure 2

(a) Islets isolated from 12-week-old male C57 mice were treated with nutlin-3a (10 μg ml−1) or DMSO as vehicle for 6 h, followed by immunoblotting using an anti-MDM2, -p53 or -β-actin antibody. Representative immunoblot images from three independent experiments are shown. The lower panel is the densitometric analysis for the relative abundance of MDM2 and p53 normalized with β-actin. (b) The islets treated with nutlin-3a or vehicle were subjected to static GSIS assay as in Fig. 1g. Note that the insulin content of islets treated with vehicle or nutlin-3a was similar. (c–f) Twelve-week-old male C57 mice were treated with nutlin-3a (10 mg kg−1) or DMSO as vehicle by oral gavage, followed by fasting for 6 h. (c) GTT. (d) Serum insulin levels during the GTT in c. (e) ITT. (f) Serum insulin levels during L-arginine stimulation test. *P<0.05 (n=6) (Student’s t-test). All data are represented as the mean±s.e.m.

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