Figure 5: Inhibition of MDM2 impairs mitochondrial metabolism in pancreatic β-cells in p53-dependent manner. | Nature Communications

Figure 5: Inhibition of MDM2 impairs mitochondrial metabolism in pancreatic β-cells in p53-dependent manner.

From: The MDM2–p53–pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells

Figure 5

Pancreatic islets isolated from 10-week-old male β-MDM2KO mice, DKO and its WT littermates were used. Intracellular levels of ATP (a), calcium influx (b), ECAR (c) and OCR (d) in the isolated islets in response to various stimuli as indicated (a,b: n=4; c,d: n=5). (e) Intracellular NADPH levels measured at 30 min after stimulation with glucose (20 mM) in the isolated islets. (f,g) The isolated islets treated with PFTα (10 μM) or DMSO as vehicle were subjected to assessment of ATP production (f) and calcium influx (g) (n=4). *P<0.05 (WT versus KO), @P<0.05 (WT+vehicle versus KO-vehicle), #P<0.05 (KO-vehicle versus KO-PFTα), **P<0.05 (Student’s t-test). All data are represented as the mean±s.e.m.

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