Figure 4: Expression (log2) comparison of MSS tumours in five classes (n=399).
From: A multigene mutation classification of 468 colorectal cancers reveals a prognostic role for APC
(a) EMT signature scores17,29, (b) RAS_AZ signature scores16, (c) the 64 β-catenin-targeted genes28 and (d) APC mRNA expression (mean of eight APC probes). Five classes: Class 0: APC wild type; Class 1: APC(1), APC(1)/KRAS, APC(1)/TP53; Class 2: APC(2), APC(2)/KRAS, APC(2)/TP53; Class 3: APC(1)/KRAS/TP53; Class 4: APC(2)/KRAS/TP53. APC (1) represents one APC mutation and APC (2) represents two APC mutations. Error bars represent s.e.m. The two-tailed, unequal variance Welch t-test was used to assess the statistical significance of comparison. Unadjusted P-values of <0.05 are shown. Notably, eight samples without suitable microarray data were excluded from 407 MSS CRCs. Class 0 tumours were significantly more ‘mesenchymal-like’ than all four other classes (a), while AKP-related Classes 3 and 4 had stronger RAS activation (b). Compared with Class 0 tumours, WNT activation, as measured by the 64 WNT target genes, was observed in APC-mutated Class 1 (P=0.0047), Class 3 (P=0.0013) and Class 4 (P=0.0018; c).
