Figure 3: Analysis of the GS-mutant tP5abcU167C. | Nature Communications

Figure 3: Analysis of the GS-mutant tP5abcU167C.

From: Visualizing the formation of an RNA folding intermediate through a fast highly modular secondary structure switch

Figure 3

(a) The GS/ES transition is abolished in the GS-stabilizing mutant tP5abcU167C. Residues disappearing or showing large differences in chemical shifts relative to WT are circled in black. Residues G164 and G176 are coloured in blue, indicating no appreciable RD. (b) Corresponding 2D 1H-15N HSQC spectrum of tP5abcU167C in 10 mM sodium phosphate and 0.01 mM EDTA (pH 6.4) at 10 °C (blue) overlaid on spectrum of WT tP5abc under the same condition (black). (c) Differential SHAPE reactivity measured on tP5abcU167C relative to WT. Red (blue) represents more (less) reactivity, respectively. The data were collected in the absence of Mg2+. (d) Off-resonance R1ρ RD profiles measured in tP5abcU167C in the absence of Mg2+.

Back to article page