Figure 1: CRISPR/Cas9 deletions in the melanin pigmentation gene Ddc result in mosaic depigmentation phenotypes in V. cardui.

(a) Location of sgRNAs relative to the V. cardui Ddc locus. Sequences of selected deletion alleles from eggs and the butterflies shown (M2, M4 and M9) confirm disruption of Ddc. Blue: sgRNA targets. Red: PAM sequences. (b) Ddc deletions result in depigmentation of larvae, including bilateral mosaics. (c) An example of mosaic melanin-specific depigmentation in adult wings.