Figure 3: Brain-specific Crmp2 cKO mice exhibit hippocampal synaptic dysfunction. | Nature Communications

Figure 3: Brain-specific Crmp2 cKO mice exhibit hippocampal synaptic dysfunction.

From: Brain-specific Crmp2 deletion leads to neuronal development deficits and behavioural impairments in mice

Figure 3

(a) cKO hippocampal slices show impaired TBS-induced LTP in acute brain slices of 8-week-old mice. (b) Summary of LTP amplitude in the last 10 min. (c) cKOs show normal basal synaptic transmission as reflected by the input–output curve. (d) The paired-pulse facilitation ratio at intervals of 60 and 500 ms in Ctrls and cKOs. (e) CRMP2 is expressed in PSD fraction of Ctrls, but not present in the PSD fraction from cKO hippocampus as analysed by western blotting. (f) Protein levels of glutamate receptor subunits GluN1 and GluN2B are reduced in the hippocampal PSD fractions from cKOs. Five micrograms of protein was loaded in each lane with β-actin as loading control and normalized to the Ctrl levels in the right panel. Data in e,f are representative of three independent experiments. Values represent mean±s.e.m. (*P<0.05; unpaired t test in b; others, one-way ANOVA with Tukey post hoc test). Scale bar, 1 mV, 5 ms (a,c).

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