Figure 1: Tsr1 is a multi-domain structure.
From: Pre-40S ribosome biogenesis factor Tsr1 is an inactive structural mimic of translational GTPases
(a) Schematic overview of Tsr1 domains, comparing the full-length protein with the Tsr1ΔNΔloop deletion mutant used in crystallization. (b) TSR1(BY4741) and pGAL::HA-TSR1 strains were transformed with plasmid pRS313 expressing the Tsr1ΔNΔloop deletion mutant or the empty vector. Following growth in galactose medium, serial dilutions of the cultures were plated on galactose medium or on medium containing glucose to repress chromosomal expression of HA-Tsr1. (c) Overview of the structure defining front and back views, related by a 180° rotation. Domains are coloured according to the schema in a. Disordered regions are indicated with dotted lines and residue numbering.
