Figure 3: WSB1 ubiquitinates LRRK2 through K27 and K29 linkages.
From: Ubiqutination via K27 and K29 chains signals aggregation and neuronal protection of LRRK2 by WSB1
(a,b) HA-tagged ubiquitin mutants with only the indicated lysine residue available for chain formation were used and an in vivo ubiquitin assay was performed. (c,d) On the basis of the results from a and b, we mutated the lysine at K27, K29 or K27 and K29 (K27R, K29R and K27–29R) to arginine and performed the in vivo ubiquitin assay. These experiments demonstrate that WSB1 ubiquitinates LRRK2 primarily through K27 and K29 lysine linkages.
