Figure 2: Medulloblastomas with amplified c-Myc oncogenes have elevated c-Myc exoRNA and exoDNA in their microvesicles.
From: Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences
(a) c-Myc amplification levels were quantified in genomic DNA (gDNA) from one normal human fibroblast line (HF19), one GBM line (11/5), one atypical teratoid rhabdoid tumour (AT/RT) line (NS224) and three medulloblastoma (MB) lines (D425, D458 and D384). ExoRNA and exoDNA were also isolated from their corresponding microvesicles. (b) qRT-PCR and (c) qPCR were carried out on nucleic acid from microvesicles from the same cell lines to measure exoRNA and exoDNA, respectively. c-Myc levels were normalized to GAPDH in the same preparation and expressed as fold increase relative to normal fibroblasts. In all cases, values are expressed as mean±s.e.m. (n=3) and analysed by two-tailed t-test comparing MB lines to HF19 (*P< 0.05, **P<0.01, ***P<0.001).
