Figure 2: MYC represses BMAL1 independent of REV-ERBα. | Nature Communications

Figure 2: MYC represses BMAL1 independent of REV-ERBα.

From: MYC/MIZ1-dependent gene repression inversely coordinates the circadian clock with cell cycle and proliferation

Figure 2

(a) Quantitative PCR (qPCR) analysis of circadian expression profiles of REV-ERBα and REV-ERBβ transcripts in synchronized U2OS t-rex tetO-MYC:V5 cells (n=3). (b) Total fluorescence and fluorescent objects quantified from synchronized U2OS t-rex Rev-VNP tetO-MYC:V5 cells (n=1). Inset: normalized number of fluorescent objects (24–96 h integration; n=2). (c) qPCR analysis of REV-ERBα and BMAL1 transcripts in siRNA-transfected U2OS t-rex tetO-MYC:V5 cells (n=3). Unsynchronized cells were collected 24 h after doxycycline induction. (d) Integrated bioluminescence of Bmal1-luc transfected U2OS t-rex tetO-MYC:V5 cells. Cells were transfected with indicated siRNAs 2 days before synchronization. Average expression levels (area under the curve) were determined over 72 h (n=3). Data are presented as mean±s.e.m. *P<0.05; two-way analysis of variance (ANOVA) with Bonferroni post-test.

Back to article page