Figure 1: Loss of Uba5 leads to death in utero because of severe anaemia.
From: The Ufm1-activating enzyme Uba5 is indispensable for erythroid differentiation in mice
(a) Morphological examination of Uba5−/− mice embryos. Representative photographs of Uba5+/+ and Uba5−/− embryos at various developmental stages. Uba5−/− embryo at E12.5 exhibited severe anaemia. Scale bar, 2 mm. (b) Haematoxylin and eosin staining of fetal liver sections of Uba5+/+ and Uba5−/− mice at E11.5. Scale bars: left panel, 1 mm; right panel, 20 μm. (c) Number of erythrocytes in fetal livers of Uba5+/+ and Uba5−/− mice at E11.5. (d) Immunohistochemistry of fetal liver sections of Uba5+/+ and Uba5−/− embryos at E11.5 with anti-ɛ-globin and anti-β-globin antibodies. Arrows: abnormal multinucleated erythrocytes. Scale bar, 20 μm. (e) Representative photographs of Uba5+/+ and Uba5−/− embryos at E11.5. The circulating erythrocyte volume is reduced in the yolk sac of Uba5−/− embryos. Scale bar, 2 mm. (f) Wright–Giemsa staining of peripheral blood cytospin cells prepared from the indicated genotypes at E11.5. Scale bar, 20 μm. (g) Percentage of abnormal erythrocytes shown in f. Bar graphs in c and g show the mean±s.d. values of five mice from each group. Statistical analysis was carried out using the unpaired t-test. *P<0.05 (Welch test).
