Figure 5: Metformin and PolyMet inhibit tumour growth by activating the AMPK and inhibiting the mTOR pathways, inducing autophagy and apoptosis mechanisms.
From: PolyMetformin combines carrier and anticancer activities for in vivo siRNA delivery
Mice bearing H460 tumours were given i.v. injections every other day and tumour proteins was prepared 24 h after the second injection for analysis. Bar charts in (a) represent quantitative analysis of normalized p-AMPK and p-mTOR band intensity using Image J. Data are shown as mean±s.d. (n=3). Cells under autophagy were stained by LC3b antibody (red) and apoptosis of cells was indicated by TUNEL assay (green). Nuclei were stained blue. Bar charts in (b) are quantitative analysis of percentage of LC3b-positive cells and the percentage of TUNEL-positive cells, respectively. Five randomly selected microscopic fields were analysed using Image J. Data are analyzed by two-way analysis of variance (ANOVA) with Tukey’s correction. Data are shown as mean±s.d. (n=5) and represent three independent experiments; NS, not significant; *P<0.05, **P<0.01, ***P<0.005. Scale bar, 200 μm.
