Figure 6: TRX f is diminished in HL on depletion of CRX. | Nature Communications

Figure 6: TRX f is diminished in HL on depletion of CRX.

From: Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast

Figure 6

MS-based 15N metabolic labelling-based quantitation from CRX (Cre03.g202950.t1.1) (a) and TRX f (Cre01.g066552.t1.1) (b). Each pie slice represents a quantified peptide. The area of a pie slice is proportional to the log2 ratio of the corresponding peptide between the indicated conditions. Colours represent pyQms quantification score; 0.7 (yellow, false discovery rate (FDR)≤1%) to 1 (blue, prefect match). (c) Selected knowledge based TRX community. The heat map represents the ratios of the proteins ranges from yellow to green indicating an upregulation and from yellow to red indicating a downregulation. The s.d. is visualized by the size of the box, smaller the box higher the s.d. of the protein ratio (see legend). For quantitative MS analysis, 14N-/15N-labelled WT and 15N-/14N-labelled IMcrx were mixed based on equal protein amount (total protein amount 100 μg). Eight different conditions were examined as followed: (1) 14N-labelled WT TAP LL versus 15N-labelled IM TAP LL, (2) 15N-labelled WT TAP LL versus 14N-labelled IM TAP LL, (3) 14N-labelled WT HSM HL versus 15N-labelled IM HSM HL, (4) 15N-labelled WT HSM HL versus 14N-labelled IM HSM HL, (5) 14N-labelled WT TAP LL versus 15N-labelled WT HSM HL, (6) 14N-labelled IM TAP LL versus 15N-labelled IM HSM HL, (7) 14N-labelled WT TAP LL versus 15N-labelled IM HSM HL and (8) 14N-labelled IM TAP LL versus 15N-labelled WT HSM HL.

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