Figure 5: Highly sensitive in vivo BLI of deep tissue tumours. | Nature Communications

Figure 5: Highly sensitive in vivo BLI of deep tissue tumours.

From: A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging

Figure 5

(a) Typical bioluminescence (BL) images of lung metastasis developed after intravenous injection of LLC/luc cells. Three-dimensional BLI (right panels) showed metastasis developed in deep lung tissues. The images were acquired after intraperitoneal injection of AkaLumine-HCl (33 mM). (b) Ex vivo BLI of metastatic lesions in the lung after removing sternum (left panel) and removed lung (right panel) of the mouse shown in a. (c) Representative BL images of LLC/luc lung metastasis (left panel) and quantitative analysis of BL production (right panel) at peak time after intraperitoneal injection of 100 μl of 33 mM substrates. n=7, *P<0.05 (t-test). (d) Representative BL images of LLC/luc disseminated in the lung (left panel) and quantitative analysis of BL production (right panel) at peak time after intraperitoneal injection of 100 μl of 5 mM substrates. n=8, *P<0.05 (t-test). Error bars indicate s.e.m. (e) Representative BL images of lung metastasis of LLC/luc (left panel) and quantitative analysis of BL production (right panel) at peak time after intraperitoneal injection of 100 μl of 5 mM substrates. n=8, *P<0.05 (t-test). Error bars indicate s.e.m.

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