Table 1 Parameters of enzymatic reaction of luciferase substrate.

From: A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging

 

K m ±s.e.m. (μM)

Vmax±s.e.m. (× 106)

D-luci

107±14.7

13.8±0.71

CycLuc1

1.06±0.06

7.00±0.09

Aka-HCl

2.06±0.63

2.07±0.23

  1. Aka-HCl, AkaLumine-HCl, CycLuc1, cyclic alkylaminoluciferin; D-luci, D-luciferin.
  2. Km and Vmax values of D-luci, CycLuc1 and Aka-HCl. These values were calculated from reactions with recombinant Fluc protein and the substrate in the presence of Mg-ATP.
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