Figure 1: Ripk3deficiency induces glucose intolerance in obese mice.
From: The necroptosis-inducing kinase RIPK3 dampens adipose tissue inflammation and glucose intolerance
Data were obtained from Ripk3 constitutive knockout mice (referred to as KO in the main text and RIPK3−/− in the figures) and WT control mice fed with a NCD or a CD-HFD for 16 weeks. Differences between WT and KO mice were determined by analysis of variance (ANOVA) with Bonferroni’s post hoc test. All data are expressed as mean±s.e.m. (a) Left panel: three-dimensional volume renderings of segmented bones (white), lungs (pink) and fat (blue) upon in vivo μCT imaging (upper panel) as well as 2D cross-sectional μCT images in transversal planes of the abdomen of the mice (lower panel). Subcutaneous and visceral fat tissue is indicated with blue arrows. Scale bar, 1 cm. Right panel: fat mass quantification (n=6 in each group) of WT and RIPK3−/− mice after 16 weeks of NCD or CD-HFD. ***P<0.001. (b) Mice were examined by glucose tolerance test (GTT). Results are expressed as mean with s.e.m., **P<0.01, ***P<0.001, n.s.: not significant. (c) Area under the curve (AUC) for the glycaemic response was calculated using the trapezoidal rule (n=5 in each group). *P<0.05, **P<0.01, ***P<0.001, n.s., not significant. (d) WT and RIPK3−/− mice fed with CD-HFD were examined at different time points 1 (n=4), 4 (n=6) and 7 (n=6) months with GTT. *P<0.05, **P<0.01, ***P<0.001.
