Figure 3: Obesity induces RIPK3 overexpression.

Data were obtained from RIPK3^−/− and WT mice fed with NCD, CD-HFD or HFD for different time points. Differences between WT and RIPK3^−/− mice were determined by Student’s t-test. All data are expressed as mean±s.e.m. (a) Protein levels of RIPK3 in the liver, skeletal muscle and epiWAT from lean and diet-induced obese mice. GAPDH was used as loading control. n.s., not specific. Western blots are representative of three independent experiments. (b) Protein levels of RIPK3 in epiWAT of mice fed for 1, 4 and 12 months with CD-HFD. (c) Upper panel: protein levels of RIPK3 in epiWAT and inguinal WAT (iWAT) from WT mice fed for 6 months with NCD or HFD. Lower panel: bar graphs for quantification of relative expression of RIPK3 in these respective tissues (n=3). *P<0.05. (d) Representative haematoxylin and eosin (H&E) images of liver (upper panel), skeletal muscle (middle panel) and epiWAT (lower panel) from WT and KO mice. Scale bars, 200 μm. (e) col1α1 mRNA levels were assessed by RT–PCR in WT and KO fed for 16 weeks after 8 weeks of methionine choline-deficient (MCD) diet feeding. Values were calculated relative to WT mice fed with NCD, and β-actin was used as an internal standard, n=6 per group. Error bars represent s.e.m.