Figure 2: Activation of NTS neurons via a Gq-coupled receptor increases firing rate in vitro. | Nature Communications

Figure 2: Activation of NTS neurons via a Gq-coupled receptor increases firing rate in vitro.

From: Genetically and functionally defined NTS to PBN brain circuits mediating anorexia

Figure 2

(a) Diagram depicting viral targeting site in a coronal section through the medial NTS of the mouse brainstem. (b) Example of spiking activity recorded from a CCKNTS neuron expressing hM3Dq:mCherry before and after addition of 3 μM CNO. (c) Application of 3 μM CNO increases firing rate in CCKNTS and DBHNTS cells expressing hM3Dq:mCherry (n=4 cells from two mice each group). No change is observed in cells expressing mCherry only (n=5 DBHNTS cells from one mouse). Effect is measured as fold change in spiking frequency from its basal level before CNO, error bars represent s.e.m.

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