Figure 4: CCKNTS or DBHNTS neuronal activation induces Fos in the NTS and in the PBN. | Nature Communications

Figure 4: CCKNTS or DBHNTS neuronal activation induces Fos in the NTS and in the PBN.

From: Genetically and functionally defined NTS to PBN brain circuits mediating anorexia

Figure 4

(a,b) Compared with animals expressing only mCherry transgene (n=6 for each genotype), CckCre/+ (a) and DbhCre/+ (b) mice expressing hM3Dq:mCherry in the NTS show increases in Fos expression in the NTS and PBN after CNO injection (unpaired t-test). Data represent mean (CCK: n=8 PBN, 9 NTS, DBH: n=6)±s.e.m. (c) Representative immunohistological sections demonstrating hM3Dq:mCherry and Fos expression in the NTS (top panels) and the PBN (bottom panels) after CNO injection. Scale bar, 100 μm. (d) Quantification of post-CNO 4-h food intake comparing individual hM3Dq low- and high-Fos-expressing animals for both the NTS and PBN (unpaired t-test). Data from CckCre/+ mice are represented by red circles, DbhCre/+ mice by black squares, lines represent mean±s.e.m., **P<0.01, ***P<0.001. AP, area postrema; cc, central canal; scp, superior cerebellar peduncle.

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