Figure 7: Determination of miR-125b-5p levels in ALF patients.

(a) Quantitative reverse transcriptase–PCR (qRT–PCR) analyses revealed elevated miR-125b-5p levels in sera of spontaneously recovered compared with non-spontaneously recovered ALF patients. *P<0.05, two-tailed Student’s t-test. (b) Decreased endogenous level of miR-125b-5p in liver biopsies obtained from ALF patients, compared with respective controls. The aetiology of ALF patients (n=8) was unknown, except for one patient who had mushroom intoxication. *P<0.05, two-tailed Student’s t-test. (c) Immunofluorescence staining for KEAP1 and NRF2 in liver sections from ALF patients and respective controls. Scale bars, 100 μm. (d) ImageJ-based quantification of staining shown in c. **P<0.01, two-tailed Student’s t-test. (e) qRT–PCR analysis of UGT1A6 and GCLC in ALF patients and respective controls. **P<0.01, two-tailed Student’s t-test. Data are presented as mean±s.e.m.