Figure 3: Identification of BLP–vCA1 structural connection in naive mice. | Nature Communications

Figure 3: Identification of BLP–vCA1 structural connection in naive mice.

From: Opposite monosynaptic scaling of BLP–vCA1 inputs governs hopefulness- and helplessness-modulated spatial learning and memory

Figure 3

(a–f) Anterograde tracing in C57BL/6 mice: AAV5–CaMKIIα–hChR2(E123T/T159C)–mCherry (red) was injected into the basolateral amygdala nucleus (BL) (a); after 6 weeks, the mCherry was predominantly shown in ventral hippocampal CA1 (vCA1) but not in dorsal CA1 viewed by serial coronal sections (b–e) and sagittal section (f). Scale bar, 1 mm. (g–s) Retrograde monosynaptic tracing in Thy1-Cre mice: the helper viruses AAV-EF1α-DIO-GT and AAV-EF1α-DIO-RV-G (1:1, 200 nl, green) was injected into vCA1, and then rabies virus EnvA-pseudotyped RV-ΔG-DsRed (red) was also injected into vCA1 (g). The BLA and BLP neurons that have monosynaptic connection with vCA1 were traced and quantified by DsRed in Thy1-Cre mice (h–k), and the BLP excitatory neurons were probed by co-staining of DsRed and anti-CaMKII in Thy1-Cre mice (p–s). The representative images of starter cells in vCA1 at helper virus injection sites (l–o). (scale bar, 1 mm (k) ;50 μm (h–j,l–s)). n=5 per group, unpaired t-test, **P<0.01 versus BLA. Data were presented as mean±s.e.m.

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