Figure 5: Stress-induced behavioural patterns are sensitive to context.
From: Hypothalamic CRH neurons orchestrate complex behaviours after stress
(a) Schematic of experiment showing the two different environments, the novel context (Novel) and footshock chamber (FS) immediately after footshock. (b) Detailed analysis shows the pattern of behaviours exhibited by the animals in different contexts. Each row represents one animal. The different environments change the behavioural pattern expressed by the animal. (c) Percentage of animals grooming at each timepoint. (d) Cumulative graphs illustrate the relative grooming in different contexts including homecage (HC) immediately after stress and compared with naïve mice (Fig. 1). (e) Grooming is the dominant in HC (dotted line represents mean grooming time in HC; Novel: 85.1±8.0 s; FS: 49.3±9.4 s; Novel versus HC P<0.0001; FS versus HC P<0.0001; n=9 in each group; one-way ANOVA). (f) Percentage of animals rearing at each timepoint. (g) Cumulative graphs illustrate the relative amount of rearing in different contexts including HC immediately after stress and the naïve mice (Fig. 1). (h) Mice spend more time rearing in Novel (dotted line represents mean rearing time in HC; Novel: 120.4±16.0 s; FS: 18.3±8.5 s; Novel versus HC P=0.0097; Novel versus FS P<0.0001; n=9 in each group; one-way ANOVA). (i) Percentage of animals walking at each timepoint. (j) Cumulative graphs illustrate the relative walking time in different contexts including HC immediately after stress and in naïve mice (Fig. 1). (k) Mice spend the same amount of time walking in Novel and FS (dotted line represents mean walking time in HC; Novel: 436.7±25.2 s; FS: 405.6±41.7 s; Novel versus HC P<0.0001; FS versus HC P<0.0001; n=9 in each group; one-way ANOVA). (l) Percentage of animals freezing at each timepoint. (m) Cumulative graphs illustrate the relative freezing in different contexts including HC immediately after stress and the naïve mice (Fig. 1). (n) Freezing behaviour was only significant in FS (dotted line represents mean freezing time in HC; Novel: 19.5±3.0 s; FS: 121.8±32.0 s; Novel versus FS P=0.0021; FS versus HC P=0.0004; n=9 in each group; one-way ANOVA). Scale bars: (c,d,f,g,i,j,l,m), 20%; **P<0.01; ***P<0.0005; ****P<0.0001; Error bars±s.e.m.
