Figure 1: Counteracting enzymes Set1 and Jhd2 function to positively or negatively co-regulate transcription in yeast. | Nature Communications

Figure 1: Counteracting enzymes Set1 and Jhd2 function to positively or negatively co-regulate transcription in yeast.

From: Counteracting H3K4 methylation modulators Set1 and Jhd2 co-regulate chromatin dynamics and gene transcription

Figure 1

(a) Strand-specific qPCR measurement of PHO5 or PHO89 sense and antisense transcription. Fold-change in transcript levels in a mutant is shown relative to WT strain (WT) (set as 1). Error bars denote±s.e.m. from three independent experiments (n=3). Statistical significance calculated using the Student’s t-test, *P-value ≤0.02, **P-value <0.001. (b) Venn diagrams showing the number of up- or down regulated sense transcripts unique to and common to jhd2Δ and set1Δ mutants. Intersection P-value <10−4 (hypergeometric test). (c) GO terms enriched in sense transcripts down regulated in both set1Δ and jhd2Δ mutants. (d) Set1 and Jhd2 either positively or negatively regulate transcription at the phosphate-responsive genes. Fold-change in total RNA-seq reads obtained in jhd2Δ or set1Δ mutant relative to WT for various phosphate-responsive genes are shown. (e) GO terms enriched in sense transcripts up regulated in both set1Δ and jhd2Δ mutants.

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