Table 1 Data collection and refinement statistics for RanGTP·Xpo4·eIF5A complex (MR-SAD).
Native | SeMet | |
|---|---|---|
Data collection | ||
Space group | P3121 | P3121 |
Cell dimensions | ||
a, b, c (Å) | 98.62, 98.62, 726.86 | 98.48, 98.48, 725.68 |
α, β, γ (°) | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 |
Resolution (Å) | 49.41−3.20 (3.31−3.20)* | 49.33−3.48 (3.61−3.48) |
Rsym | 0.08 (0.95) | 0.15 (1.96) |
I/σI | 22.42 (2.26) | 18.00 (1.40) |
Completeness (%) | 99.91 (99.79) | 99.70 (97.10) |
Redundancy | 9.90 (10.20) | 18.70 (15.10) |
Refinement | ||
Resolution (Å) | 49.41−3.20 | – |
No. reflections | 69,887 | – |
Rwork/Rfree (%) | 21.5/26.8 | – |
No. atoms | ||
Protein | 20,622 | – |
Ligand/ion | 66 | – |
Water | 0 | – |
B-factors | ||
Protein | 118.88 | – |
Ligand/ion | 75.92 | – |
Water | – | – |
r.m.s. deviations | ||
Bond lengths (Å) | 0.006 | – |
Bond angles (°) | 0.57 | – |
