Figure 5: V2 cell eccentricity polarizes the localization of DeltaC protein.
From: Memory of cell shape biases stochastic fate decision-making despite mitotic rounding
(a) Quantitative measurement of DeltaC protein distribution. (b) Polarized localization of DeltaC protein on the (+) side of V2 cell. (c) Diffusion-like behaviour of the initially polarized DeltaC::mCherry fusion protein during mitotic cell rounding. (d) Translocation of DeltaC::mCherry fusion protein on changes of the direction of the asymmetric elongation of V2 cell shape. The spiky ends of the old (yellow arrow) and new (orange arrow) axes are indicated. Irradiation at the spiky ends of the old axis (yellow arrow) altered the asymmetry orientation generating the new axis. Biased localization of the DeltaC::mCherry fusion protein is shifted according to the newly acquired orientation of V2 cell shape asymmetry. *P<0.05, **P<0.01 (χ2-test). Scale bars, 5 μm (b), 10 μm (c,d). See also Supplementary Fig. 4 and Supplementary Movies 5 and 6.
