Figure 1: Experimental set and probes’ cellular responses.
From: Development of background-free tame fluorescent probes for intracellular live cell imaging
(a) Flowchart of the overall experimental strategy. (b) Representative cellular responses in CHO and U-2 OS. Cells were stained with probes at 1 μM final concentration. The overlayed images of Hoechst33324 (blue) and probe signals (according to emission wavelength of the probes) show three phenotypic groups: cell-impermeable group where probes are unable to stain the cell (N-group); cell permeable with low nonspecific binding group (L-group); and cell permeable with high nonspecific binding group (H-group). BW and AW indicate image before and after washing, respectively. Probes signal is significantly decreased after washing in L-group, while is retained in H-group. Scale bar, 10 μm (green, red and blue signals are from FITC, Texas Red and DAPI channel, respectively). (c) Core structures of the BODIPY libraries used in training set.
