Figure 2: Mechanical properties of talin.
(a) Unfolding force-extension curves of FL-talin rod at the loading rate of 3.8 pN s−1. The different curves represent repeated force cycles from a single protein tether (inset: the R3 unfolding step occurs at 5 pN; scale bar, 1 s). The data were smoothed by 0.05 s time window for clearer presentation. (b) The unfolding force histogram and corresponding unfolded contour length of FL-talin rod constructs (data from three independent tethers) under constant loading rates of 3.8 pN. The unfolding signals are grouped into four groups I–IV based on the distinctive unfolding forces.
