Figure 6: Simulation of force in talin-mediated force transmission pathway.
(a) Illustration of the in vivo talin extension (left panel) and the measured distribution of talin extension in fibroblast cells15. (b) Simulation of the force (top panel) and the number of unfolded domains (middle panel) during changing the FL-talin extension as indicated in bottom panel. (c) Average force (data in blue) and number of unfolded domains (data in red) in FL-talin rod as a function of extension. Solid connecting lines are provided for visual guiding. The error bars denote s.d.’s. The black box denotes the physiological range of extension measured in fibroblast cells as shown in a. (d,e) Simulated force fluctuations (top panels) in FL-talin based on two different levels of FL-talin extension fluctuations around (d) 100 and (e) 200 nm recorded from living cells15. Talin end-to-end fluctuations measured experimentally from in vivo single-molecule localization studies15 in fibroblasts are shown in the third panels. First panels: the estimated force fluctuation on the talin rod. Second panels: NVBS denotes the number of exposed VBS. Bottom panels: heat maps showing the unfolding probability of each individual talin rod domain during the time evolution. The talin was significantly more extended in e than in d.
