Figure 2: Site-directed spin-labelling analysis of the MscL N terminus. | Nature Communications

Figure 2: Site-directed spin-labelling analysis of the MscL N terminus.

From: The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels

Figure 2

(a) X-band CW-EPR spectra of spin-labelled N-terminal mutants reconstituted into azolectin liposomes. Spectra are colour-coded according to their overall probe dynamics as shown in the colour gradient (bottom right). (b) Environmental parameter profiles derived from the spectra in a or from power saturation experiments. Mobility parameter ΔHo−1 (top panel, black circles), oxygen-accessibility parameter ΔO2 (centre panel, red squares) and NiEdda-accessibility parameter ΔNiEdda (bottom panel, blue triangles). A cartoon of the assigned secondary structure for this segment is shown on top. Bar represents 20 G. (c) EPR-derived structural data mapped on MtMscL. The side and top (extracellular) views of the mapped EPR data are displayed on solvent-accessible surfaces calculated in UCSF Chimera with a probe radius of 1.4 Å. Green tones, probe mobility (ΔHo−1). Red tones, oxygen-accessibility parameter (ΔO2). Blue tones, NiEdda-accessibility parameter (ΔNiEdda). The dotted parallel lines represent the putative location of the lipid bilayer.

Back to article page