Figure 5: Conformational changes at the N terminus associated with the channel opening. | Nature Communications

Figure 5: Conformational changes at the N terminus associated with the channel opening.

From: The role of MscL amphipathic N terminus indicates a blueprint for bilayer-mediated gating of mechanosensitive channels

Figure 5

(a) X-band CW-EPR spectra of liposome-reconstituted spin-labelled N-terminal mutants in the presence (red traces) and in the absence (black traces) of 25 mol% LPC to stabilize the open conformation. Experimental conditions as described in Fig. 3. Bar represents 30 G. (b) Environmental parameter profiles of the N terminus in the open state, derived from the spectra in a or from power saturation experiments. Mobility parameter ΔHo−1 (top panel, black circles), oxygen-accessibility parameter ΔO2 (centre panel, red squares) and NiEdda-accessibility parameter ΔNiEdda (bottom panel, blue triangles). For comparison, data for the closed state (from Fig. 2) are shown on each panel as grey open symbols. The vertical dashed lines point to the periodic maxima of NiEdda-accessibility and O2-accessibility minima in the open state.

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