Figure 5: Localization of EhC2PK and pan membrane protein EhTMKB1-9 in cells undergoing erythrophagocytosis.
From: A C2 domain protein kinase initiates phagocytosis in the protozoan parasite Entamoeba histolytica
(a) Amoebic cells undergoing erythrophagocytosis stained for EhC2PK and TMK-9 (phagocytic cup marked by arrow). (b) A newly formed phagocytic cup (marked 1) and a phagosome (marked 2). (c) Intensity of immunostain (EhC2PK, EhTMK-9) was measured at multiple locations in the membrane and average relative intensity was computed by taking the signal from membrane as 100% for each marker separately. Quantitative estimation of immunostained signals in phagosomes compared with the phagocytic cups and the cytoplasm is shown. EhC2PK and TMK-9 are stained by Alexa488 and Alexa546, respectively (green, cytosol; red, phagocytic cups; blue, phagosome). For c, five random regions were selected from membrane, cytosol and phagocytic cups and average intensity was computed for each region. This was repeated for four such cells (N=4, bars represent standard error; scale bar, 10 μm ; DIC, differential interference contrast).
