Figure 6: SARI inhibits expression of downstream targets in vivo. | Nature Communications

Figure 6: SARI inhibits expression of downstream targets in vivo.

From: RETRACTED ARTICLE: SARI inhibits angiogenesis and tumour growth of human colon cancer through directly targeting ceruloplasmin

Figure 6

(a) Staining for VEGF (red) revealed less VEGF-positive cells in SARI than in controlSW480Matrigel plugs, Scale bar, 50 μm; VEGF-positive (%; VEGF positive per total cells; n=5; **P<0.01; Student’s t-test). (b) Staining for VEGF (red) revealed less VEGF-positive cells in SARI than in control HCT116 tumours (d), Scale bar, 100 μm; VEGF positive (%;VEGF positive per total cells; n=5; **P<0.01; Student’s t-test). (c) Staining for Cp revealed less Cp-positive cells in SARI than in control HCT116 tumours, Scale bar, 100 μm; Cp positive (%;Cp positive per total cells; n=5; **P<0.01; Student’s t-test). (d) Staining for HIF-1α (red)-positive cells in SARI and control HCT116 tumours, Scale bar, 100 μm; HIF-1α positive (%; HIF-1α positive per total cells; n=5; **P<0.01; Student’s t-test). (e) Immunoblots of VEGF, Cp and HIF-1a expression in SW480-control and SW480-SARI tumours. GAPDH was used as the loading control. (f) ELISA detection of VEGF expression in SW480-control and SW480-SARI tumours (n=3; **P<0.01; Student’s t-test). (g) Staining for CD31, VEGF, Cp and HIF-1α in colonic tumours of AOM/DSS induction. Analysis of the number of microvessels, VEGF-positive cells, Cp-positive cells and HIF-1α-positive cells (n=4; **P<0.01; Student’s t-test). Scale bar, 100 μm. (h) Immunoblots of VEGF, Cp and HIF-1α expression in colonic tumours of SARI−/− and SARIWT mice after AOM/DSS induction. GAPDH was used as the loading control. (i) ELISA detection of VEGF expression in colonic tumours of SARI−/− and SARIWT mice after AOM/DSS induction. (n=3; **P<0.01; Student’s t-test) (j) Nuclear and cytoplasmic proteins were collected from the colonic tumours of SARI−/− and SARIWT mice after AOM/DSS induction. Western blotting was performed to detect Cp expression. Histone H3 was used as the nuclear loading control and β-actin as the cytoplasmic loading control.

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