Figure 4: RNA interaction sites.
From: Mapping RNA–RNA interactome and RNA structure in vivo by MARIO
(a) Multiple MARIO reads, representative of different interactions (dashed lines), overlapped on specific regions of the Eef1a1 gene. (b) Finding interaction sites by the ‘peaks’ of overlapping reads. (c) Distribution of interaction sites in different types of RNA genes and transposons. (d) The distribution of binding energies (ΔG, kcal mol−1) between the interaction sites of two RNAs (grey) and between randomly shuffled bases (white). P-values from Wilcoxon rank test are marked at the bottom of each panel. (e) Conservation levels, measured by average PhyloP scores peaked at the junction (black bar, position 0 on the x axis) of the ligated RNA fragments. Control: conservation levels of randomly selected genomic regions.
