Figure 2: PACAP and PAC1-R expression in wild-type male mouse infraorbital lacrimal gland.
From: PACAP suppresses dry eye signs by stimulating tear secretion
(a) Reverse transcriptase PCR analysis to detect Adcyap1 and Adcyap1r1 mRNA in the infraorbital lacrimal gland. The eye ball was used as a positive control. (b–d) PACAP immunoreactivity in the mouse infraorbital lacrimal gland with NeuN immunoreactivity as a neuronal marker (b,c), and ChAT immunoreactivity as a marker of parasympathetic neurons (d). The white square in b is expanded in c. Scale bar, 20 μm in b and c and 10 μm in d. (e,f) PAC1-R immunoreactivity in the infraorbital lacrimal gland identified with single immunostaining in the acinus (arrow head) and in the duct (arrow) (e), and with smooth muscle actin (SMA) immunoreactivity as a myoepithelial cell marker (f). Scale bar, 20 μm in e and 5 μm in f. DAPI was used as a nuclear marker (e,f).
