Figure 7: PACAP eye drop-induced phosphorylation and trafficking of AQP5.
From: PACAP suppresses dry eye signs by stimulating tear secretion
(a) Representative images of AQP5 immunoreactivities in infraorbital lacrimal gland acinar cells after the application of saline, PACAP38, PACAP38+SQ22536 (SQ) or PACAP38+PACAP6–38 eye drops. Scale bar, 10 μm. (b) AQP5 signals in fractionated infraorbital lacrimal glands and the semi-quantified results (n=11 per group). **P<0.01. (c) AQP5 signals in total lysate and immunoprecipitation with a pan-phospho antibody. Immunoprecipitation with a normal IgG antibody was used as a negative control. (d,e) AQP5 signals in fractionated infraorbital lacrimal glands (d) and semi-quantified results (e) (n=11 animals per group). *P<0.05
