Figure 8: Contribution of AQP5 to PACAP-induced tear secretion in mice.
From: PACAP suppresses dry eye signs by stimulating tear secretion
(a) Experimental design of AQP5 siRNA study. (b–d) AQP4 and AQP5 levels in the infraorbital lacrimal glands of male wild-type mice 1 day after AQP5 siRNA treatment. Aqp4 (b) and Aqp5 (c) mRNA levels were measured by real-time PCR (n=12 per group). *P<0.05 (d) Representative pictures of AQP4 and AQP5 immunoreactivities in the infraorbital lacrimal gland after control and AQP5 siRNA injection. Scale bar, 20 μm. (e) Effect of AQP5 siRNA treatment of infraorbital lacrimal glands on PACAP-induced tear secretion (n=12). *P<0.05, **P<0.01. (f) Schema of proposed mechanisms of PACAP-induced tear secretion.
