Figure 5: 2-Br-palmitate(2BP) and palmitic acid(PA) treatments alter Nav1.5 biophysical properties in HEK293 cells.

2-Br-palmitate treatment group data are shown in green and palmitic acid treatment group data are shown in blue. Control conditions are shown in black lines and open symbols. All data are shown as mean±s.e.m. (a,b) Left: normalized currents of Nav1.5 compared with 2-Br-palmitate/palmitic acid treatments. Channels were depolarized to −20 mV from a holding potential of −100 mV. Middle: voltage dependence of activation and steady-state inactivation of the channel. Right: Nav1.5 recovery from inactivation. n=11. (c,e) 2-Br-palmitate and palmitic acid treatment does not affect Nav1.5 voltage dependence of slow inactivation ((c)10 s; (e)30 s; n=8). (d,f) 2-Br-palmitate and palmitic acid treatment does not affect Nav1.5 recovery from slow inactivation ((d)10 s; (f) 30 s; n=8).