Figure 4: m5C34 in mt-tRNAMet is a necessary precursor for f5C34.
(a) Heatmaps of BS RNA-Seq reads for mt-tRNAMet (MT-TM) for wt/wt and mut/mut cells, showing cytosines of individual reads (on y axis). Methylated (and hydroxymethylated) cytosines are shown in orange, while unmodified or formylated cytosines are shown in grey (x axis). (b) Heatmaps of fCAB RNA-Seq reads for mt-tRNAMet (MT-TM) for wt/wt and mut/mut cells, showing cytosines of individual reads (on y axis). Purple indicates methylated, hydroxymethylated or formylated, while unmodified cytosines are shown in grey. (c) Heatmaps of RedBS RNA-Seq for mt-tRNAMet (MT-TM) for wt/wt and mut/mut cells showing cytosines of individual reads (on y axis). Purple indicates methylated, hydroxymethylated or formylated, while unmodified cytosines are shown in grey. (d) Summary of the fCAB RNA-Seq results for wt/wt and mut/mut cells. (e) Summary of the RedBS RNA-Seq results for wt/wt and mut/mut cells. (f) Graphical overview of the mt-tRNAMet C34 formylation pathway. NSun3 methylates unmodified C34 into m5C34, which is then further processed (responsible enzyme(s) unknown) into f5C34.
