Figure 2: WARS2 regulates endothelial cell morphology and angiogenic potential. | Nature Communications

Figure 2: WARS2 regulates endothelial cell morphology and angiogenic potential.

From: Wars2 is a determinant of angiogenesis

Figure 2

(a) Bright field micrographs of endothelial cells (ECs) following transfection with either control siRNA (siNT) or siRNA against WARS2 (siWARS2). Scale bar=200 μm. (b) Confocal microscopy of ECs transfected with siNT or siWARS2 (red, mitochondria; green, actin; blue, nucleus; scale bar =30 μm). (c) EC number in EC cultures transfected with siNT or siWARS2 (n=3 per condition, t-test). (d) Super-resolution microscopy of ECs transfected with siNT or siWARS2 and stained for actin (left), mitochondria (middle) and composite images with nuclear stain (right). Scale bar =25 μm. (e–j) Effects of WARS2 in an in vitro model of EC angiogenesis. (e–g) WARS2 loss of function; (h–j) WARS2 gain-of-function. Total tubes (e, h), total tube length (in pixel) (f, i) and total branching points (g, j). n=8, t-test. **, P<0.01; ***, P<0.001.

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