Figure 5: Immune cell composition of normal colonic mucosa of syCRC and soCRC patients. | Nature Communications

Figure 5: Immune cell composition of normal colonic mucosa of syCRC and soCRC patients.

From: Patients with genetically heterogeneous synchronous colorectal cancer carry rare damaging germline mutations in immune-related genes

Figure 5

(a) Percentages of CD8+ T cells over all CD3+ cells in the normal mucosa and lamina propria of syCRC and soCRC patients. Distributions were compared using the one-tailed Wilcoxon rank-sum test. (b) Example of immunostaining for CD8 (Novocastra, Clone 4B11) and CD3 (Novocastra, Clone LN10). Dotted lines mark the boundary between the epithelium and lamina propria. Arrowheads indicate intraepithelial lymphocytes in the surface epithelium. Magnification 200 × ; scale bar, 30 μm. (c) Percentages of CD3+ T cells in the surface epithelium and colon crypts of syCRC and soCRC patients. The number of samples is different in the two comparisons because not all types of information were available for all samples. Distributions were compared using the one-tailed Wilcoxon rank-sum test. (d) Example of immunostaining for CD3 cells with a blue nuclear counterstain. A representative area used to score frequency of surface intraepithelial lymphocytes is boxed and included as a magnified insert in the case of syCRC. Original magnification, 200 × ; scale bar, 30 μm. For each distribution, reported are the median value (horizontal line) and 1.5 times the interquartile ranges (whiskers).

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