Figure 6: Flag–SRSF3-WT but not K85R mutant can rescue the SG assembly defect in SRSF3-depleted cells. | Nature Communications

Figure 6: Flag–SRSF3-WT but not K85R mutant can rescue the SG assembly defect in SRSF3-depleted cells.

From: NEDDylation promotes stress granule assembly

Figure 6

(a) Stably expressing empty vector, Flag–SRSF3-WT or K85R mutant cells transfected with siCONT or siSRSF3-3′UTR were treated with 0.2 mM arsenite before processing for immunofluorescence microscopy using anti-G3BP and anti-Flag antibody. Percentage of cells bearing SGs in (b) siCONT-transfected cells and (c) siSRSF3-3′UTR-transfected cells are shown as bar graph. Error bars indicate s.e.m. (n=4). **P<0.01, Student’s t-test. (d) Knockdown efficiency of endogenous SRSF3 and the expression Flag constructs were assessed by western blot analysis. Scale bar, 10 μm.

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