Figure 1: CLIP-seq reveals that TAF15 binds GGUAAGU motifs in the mouse brain. | Nature Communications

Figure 1: CLIP-seq reveals that TAF15 binds GGUAAGU motifs in the mouse brain.

From: Distinct and shared functions of ALS-associated proteins TDP-43, FUS and TAF15 revealed by multisystem analyses

Figure 1

(a) Autoradiograph of TAF15 protein–RNA complexes from the mouse brain immunoprecipitated with an antibody against TAF15 (left panel). RNA residing in the regions outlined by the red boxes was recovered for sequencing. TAF15–RNA complexes migrated at the expected size and were efficiently recovered because little protein remained in post-immunoprecipitation lysate (right panel, middle lane). (b) Flow chart illustrating CLIP-seq reads analysed to define TAF15 clusters. (c) De novo sequence motifs enriched above background within the transcriptome or specific genic regions with associated binomial P values. (d) Positional distribution of the TAF15 motif GGUAAGU within TAF15 (red), FUS (green) or TDP-43 (purple) CLIP clusters. Inset graph shows the per cent enrichment of the TAF15 motif GGUAAGU within TAF15 targets (‘Target’) or within the transcriptome (‘Background’). (e) Positional distribution analysis of the TAF15 motif GGUAAGU as in d but specifically within CLIP clusters residing in 3′ UTRs. Inset graph shows the per cent enrichment of the TAF15 motif GGUAAGU within the 3′ UTRs of TAF15 targets (‘Target’) or within the 3′ UTRs of the transcriptome (‘Background’).

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