Figure 2: LPAATδ is a trans-Golgi–localized enzyme. | Nature Communications

Figure 2: LPAATδ is a trans-Golgi–localized enzyme.

From: Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ

Figure 2

(a) Representative confocal microscopy image of HeLa cells at steady-state fixed and labelled with a polyclonal anti-LPAATδ antibody (endogenous LPAATδ; red), with a monoclonal anti-GM130 antibody (green) and with an anti-TGN46 antibody (blue). Inset, bottom: Magnification of Golgi area. (b) Line scan across the Golgi area (red line across the magnified image in a) indicates the colocalization of LPAATδ with TGN46. (c) Representative confocal microscopy images of COS7 cells at steady-state (top) or VSV-infected and subjected to the VSVG TGN-exit assay (bottom). Cells were fixed and labelled with a polyclonal anti-LPAATδ antibody (endogenous LPAATδ; green) and with an anti-TGN46 antibody (red; top) or a monoclonal anti-VSVG antibody (red; bottom). Inset, right: magnification of tubular carrier precursors. Scale bars, 10 μm (a,c).

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