Figure 1: Gating strategy for the identification of mouse MDSC subsets.
From: Recommendations for myeloid-derived suppressor cell nomenclature and characterization standards

Gating strategy used to define MDSC subpopulations in BM, blood and spleen of C57Bl/6 tumour-free or MCA203 tumour-bearing mice. After exclusion of doublets (not shown), live CD11b+ cells were gated and the proportion of Ly6C and Ly6G cells was evaluated.