Figure 2: Gating strategy for the identification of MDSC subsets in the peripheral blood of healthy donors and melanoma patients. | Nature Communications

Figure 2: Gating strategy for the identification of MDSC subsets in the peripheral blood of healthy donors and melanoma patients.

From: Recommendations for myeloid-derived suppressor cell nomenclature and characterization standards

Figure 2

Doublets were excluded and live PBMC were gated (not shown). (a) CD14+HLA-DR−/lo M-MDSC. Monocytes were gated on the basis of FSC and SSC parameters and HLA-DR downregulation was defined by FMO control. (b) Lin−HLA-DR−CD33+ eMDSC. (c) CD14−CD15+CD11b+ PMN-MDSC.

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