Figure 4: ERRα restores the glutamine flux in lapatinib-resistant cells.
From: ERRα mediates metabolic adaptations driving lapatinib resistance in breast cancer
Schema representing the flux of 13C5-labelled glutamine through the citric acid cycle in forward and reverse directions. Isotope-labelled carbon is shown in red, and total change in metabolite mass is indicated. Bar charts show quantification of the fraction of intermediate metabolites produced from the flux of 13C5-labelled glutamine in pSKBr3 and LRSKBr3 cells on lapatinib treatment and pharmacological inhibition of ERRα with C29. Fraction of labelled intermediate metabolites issued from the TCA cycle is detected for both the forward (outside box) and reverse (inside box) cycle flux. Each graph represents three independent experiments performed in triplicate, error bars are s.e.m., statistical significance is calculated using two-tailed unpaired t-test; *P<0.05; **P<0.01; ***P<0.001. Western blot depicts the expression of ERRα in pSKBr3 and LRSKBr3 cells on 24-h lapatinib and/or C29 treatment.
