Figure 2: Chromosomal aberrations are a late event in breast cancer tumorigenesis and further aberrations are acquired during the derivation of cell lines. | Nature Communications

Figure 2: Chromosomal aberrations are a late event in breast cancer tumorigenesis and further aberrations are acquired during the derivation of cell lines.

From: The landscape of chromosomal aberrations in breast cancer mouse models reveals driver-specific routes to tumorigenesis

Figure 2

(a) Following chromosomal aberrations in the SV40Tag mouse model (GSE21444) reveals that large CNAs characterize the progression of non-malignant lesions to invasive carcinomas. Presented are moving average plots of gene expression profiles from various stages of tumour development. (b) Quantification of the prevalence of chromosomal aberrations in normal tissues (0/52), premalignant tissues (1/51) and invasive carcinomas (68/96) derived from the SV40Tag mouse model. *P=1.7 × 10−15 and *P<1 × 10−16 (χ2-test) for the comparison of tumours to normal and to premalignant tissues, respectively. (c) Following chromosomal aberrations in the PyMT mouse model (GSE43566) reveals that metastasis is not associated with an increased burden of aneuploidy and large CNAs. Presented are moving average plots of gene expression profiles from primary tumours (left; one aberration detected in 11 samples), and from disseminated cells and metastases (right; two aberrations detected in 22 samples). (d) Quantification of the prevalence of chromosomal aberrations in primary tumours (n=1,699), freshly derived cell lines (n=17), established cell lines (n=56) and cell line-derived tumours (n=30), revealing that cell lines exhibit an increased degree of chromosomal instability. *P=2 × 10−10, *P<1 × 10−16 and *P=2 × 10−11 (χ2-test) for the differences between primary tumours and freshly derived cell lines, established cell lines and cell line-derived tumours, respectively. Single, 1 CNA detected; multiple, >1 CNA detected. (e) Box plots presenting the number of arm-level CNAs in human primary breast tumours (from The Cancer Genome Atlas) and in human breast cancer cell lines (from the Cancer Cell Line Encyclopedia), divided by molecular subtype. *P=1.2 × 10−4 and *P=9.7 × 10−7 (Student’s t-test) for the luminal subtype and for all subtypes combined, respectively. Boxes show the median, 25th and 75th percentiles, lower whiskers show data within 25th percentile −1.5 times the IQR, upper whiskers show data within 75th percentile +1.5 times the IQR and circles show outliers. See also Supplementary Figs 2–4.

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