Figure 1: Mass spectrum of Fdc1 and Fdc1Ubix displaying stabilising effects of the prFMN cofactor. | Nature Communications

Figure 1: Mass spectrum of Fdc1 and Fdc1Ubix displaying stabilising effects of the prFMN cofactor.

From: Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding

Figure 1

(a) Native MS of 5 μM Fdc1 and (b) 10 μM Fdc1UbiX showing some dissociation of the Fdc1 dimer into monomer, while all of the Fdc1UbiX is present as a dimer. This figure has been adapted from Fig. 5b in the extended data set of Payne et al.2 here, reporting data over a wider m/z range. Both samples were sprayed from 100 mM ammonium acetate, pH 6.8. Right hand spectrum; an enlarged view of [2M+21H]21+. The measured mass of dimeric Fdc1 is 112,266.4±1.5 Da (expected 112,270 Da). The predicted masses are shown by blue dashed lines.

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